Spectrophotometer

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Model No: HO-SP-3480

The HO-SP-3480 is a microprocessor controlled Visible Range Spectrophotometer covering the wavelength range of 340 to 800nm with 10nm bandwidth. The monochromator is of a Czerny Turner design, incorporating a stepper motor driven 1200 lines/mm holographic diffraction grating. It has full interfacing capability for Analog output and serial (RS232) interfacing.

Though this system is designed to operates in the wavelength range of 340 to 800 nm, special gratings and detectors are available for increasing the spectral range and efficiency. It uses blazed holographic grating as dispersive element and the optimized Czerny Turner-type Monochromator to reduce stray light, and widen the photometric range. Various accessories are available to address micro-volume samples and individual application requirements.

The amount of light passing through the tube is measured by a photometer. The photometer delivers a voltage signal to a processing unit. The signal changes

as the amount of light absorbed by the liquid changes. According to Beer's law, the amount of light absorbed by a medium is proportional to the concentration of the absorbing material or solute present. Thus the concentration of a colored solute in a solution may be determined in the lab by measuring the absorbency of light at a given wavelength.

The computer system is used to plot a graph of intensity vs wavelength. or the wavelength scans in absorbance or %Transmittance. Acquired scan data are stored and may be used for various analysis and calculations. Includes the calculation mode to add, subtract, multiply and divide spectra.

Wavelength
Range	:	340 - 800nm
Resolution	:	1 nm
Accuracy	:	± 2nm
Bandwidth	:	10 nm
Transmittance
Range	:	0 to 199.9 %
Resolution	:	0.1 %
Stray light	:	<0.5 %
Photometric accuracy	:	± 2 %
Absorbance
Range	:	0.300 to 1.999A
Resolution	:	0.001A
Concentration
Range	:	300 to 1999 Concentration
Resolution	:	0.1/1
Factor	:	0 to 999.9, 1000 to 9999
Photometric Noise Levels	:	<1%
Photometric Stability	:	1% / Hr after warm-up
Readout	:	LCD Graphics display
Outputs	:	Analogue (0-1.999VDC.) RS232 serial port
Light Source	:	Tungsten Halogen 20W 12V

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Model HO-SP-3480 has 1 mm, 2 mm, 5 mm and 10 mm pathlength cuvettes. These Cuvettes are manufactured from high-quality optical glass materials.

For most (99%) absorbance measurements 10 mm pathlength cuvettes are suitable. Cuvettes of shorter pathlength allow the measurement of concentrated samples without dilution.

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