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Digital Inline Holography Microscope

Model No: HO-DIHM-T01

Holmarc’s Digital In-line Holography microscope, model HO-DIHM-T01, is a powerful imaging device that allows for the digital reconstruction of complex objects (amplitude and phase) from a single intensity image captured by passing coherent light through it.

Holmarc’s DIHM is equipped with a transmission type in-line geometry. A coherent point light source illuminates the sample, and a CMOS camera records the 2D interference pattern created by the scattered light (object beam) with non-scattered light (reference beam). The software then computes the field backward to different planes to numerically recreate the samples’ 3D structure from the recorded 2D interference pattern. For 3D reconstruction, the software employs a single shot phase unwrapping algorithm. The DIHM’s broad depth of field allows it to image large volumes with equivalent resolution and to image particles at different depths simultaneously without the use of mechanical scanning.

The HO-DIHM-T01 can be used both in-situ and ex-situ to image particles ranging in size from sub-microns to several millimetres. It records the interference pattern using a 405nm diode laser with pinhole assembly as a point light source and Sony’s 1/1.8” 3.17Mpix CMOS IMX174 sensor. The 134 fps USB3.0 sensor delivers great image quality and low noise performance. The distance between the sample to pinhole and sample to camera is adjustable from the software. A motorized ND filter wheel is provided in front of the laser to adjust the laser power for different sample conditions. DIHM_V01 is an easy-to-use programme that allows you to save, rebuild, and analyse each hologram frame.


    DIHM Type : Transmission, Upright Geometry
    Light source : 405nm diode laser, 50 mW
    Point source assembly : Microscope objective + pinhole assembly
    Software : DIHM_V01
    Phase unwrapping : Single shot algorithm
    Camera : 1/1.8”, 3.17MP, CMOS, Mono USB3.0, SONY, Global Shutter
    Pinhole - sample distance : Adjustable, motorized, controls via software
    Sample - Camera distance : Adjustable, motorized, controls via software
    Laser power : Adjustable via motorized ND filter
    Particle size analysis

    Particle tracking in 3D

    Cell biology, 3D dynamic analysis of cells

    Study of ice crystal habits in could chamber

    Microfluidics

    Marine biology

    Deformation analysis

    Refractive index characterization

    Ferroelectric metrology

    Electro-chemistry

Fig. 1. Hologram - Single frame


Fig. 2. Binary particle map


Particle 1 - Amplitude


Particle 1 - Phase


Particle 1 - 3D Phase

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Particle 2 - Amplitude

Particle 2 - Phase

Particle 2 - 3D Phase

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Particle 3 - Amplitude

Particle 3 - Phase

Particle 3 - 3D Phase

-

Particle 4 - Amplitude

Particle 4 - Phase

Particle 4 - 3D Phase

-

Particle 5 - Amplitude

Particle 5 - Phase

Particle 5 - 3D Phase

-

Particle 6 - Amplitude

Particle 6 - Phase

Particle 6 - 3D Phase

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